The following compounds do not cross-react with (5O ng/ml cut-off)

3.2   cocaine   (BEG) 300 ng/ml Cut-Off Level

Marijuana at a 100 ng/ml concentration in urine:

Acelaminophen

digitoxin

meperidine

                                        Svva EMIT II Positive   Svva EMIT II Negative

4-Aceiamitlaphenul

digoxin

metadone

QuikScreen"  Positive                    165                                   2

QuikScreen*  Negative                     0                                   151

Acelylsalicylic Acid

Ecgonine -HCL

methaqualone

Amikacin

Ecgonine Methyl Ester

naloxone

Ampicillin

ephedrine

neomycin

D,l-Amphetamine

epinephrine

niacinamide

When compared to EMIT II the relative sensitivity   between   positive samples was 100% . The relative specificity   between negative samples was 98.69%.

Amilriptyline

Gentistic Acid

oxazepam

arlerenol

glucose

perphenazine

Asparlame

guaiacol

Penicillin G

The concordance of the combined data with respect to EMIT II

Atropine Sulfate

Glyceryl Ether

phencyclidine

was 99.37%.

Benzoic Acid

histamine

phenobarbital

benzoylecgonine

hydrochlorothiazide

a-Phenylethylamine

3.3   marijuana    (THC) 50 ng/ml Cut-Off Level

Caffeine

hydrocodone

phenylpropanolamine

camphor

hydromorphone

promethazine

chloroquine

homalropine

pseudoephedrine

                                         Svva EMIT II Positive   Svva EMIT II Negative

chlorpheniramine

homairopine

ranitidine

Chlorpromazine –HCL

imipramine

Salicylic Acid

QuikScreen* Positive                      52                                   0

Cocaine -HCL

isoproterenol

secobarbital

QuikScreen*  Negative                    0                                   513

cocaine

ketamine

tetracycline

cimetidine

lidocaine

tetrahydrozoline

cortisone

methylphenidate

theophylline

When compared to EMIT 11 the relative sensitivity   between   positive

deoxyepinephrine

Morphine-3-B-D-Glucuronide

thioridazine

samples was 100% . The relative specificity  between negative samples was

dextromethorphan

Morphine sulfate

Trifluoperazine

100%. The concordance of the combined data with respect to EMIT II was

diazepam

d-methamphetamine

tryptophan

100%.

The following compounils do not cross-react with  (2000 ng/ml cut-off) Opiates at a   100 fig/ml concentration in urine:

3.4   opiates   (OPI) 2000 ng/ml Cut-Off Level

acetaminophen

5,5-Diphenythdantion

pentobarbital

Acetylsalicylic Acid

doxylamine

d-Propoxyphene

                                        Svva EMIT II Positive   Svva EMIT II Negative

amikacin

Ecgonine - HCL

1-propanol

amitriptyline

Ecgonin Methyl Ester

phencyclideine

QuikScreen⁸  Positive                     195                                     0 QuikScreen* Negative                     0                                   500

ampicillin

glucose

phenobarbital

When compared to EMIT II the relative sensitivity   between   positive

arterenol

histamine

phentermine

samples was 100% . The relative specificity  between negative samples was

aspartame

hydrochlorothiazide

phenylpropanotamine

100%. The concordance of the combined data with respect to EMIT II was

Benzoic Acid

indomethacin

1-Phenylephrine

100%.

benzoylecgonine

ketoprofen

quinine

caffeine

∆9- THC

Sodium Salicylate

3.5 phencvclieine    (PCP)   25ng/ml Cut-Off Level

chlorpheniramine

11-Nor-∆9-THC-9-COOH

tryptophan

                                        Svva EMIT II Positive   Svva EMIT II Negative

chlorpromazine

methylphenidate

tetracycline

cimetidine

methadine

tetrahydrozoline

QuikScreen*'   Positive                      14                                          0

deoxyephedrine

methaqualone

theophylline

dextromethorphan

oxazepam

thioridazine

QuikScreen*  Negative                      0                                         551

diazepam

phendimetrazine

trifluoperazine

When compared to EMIT II the relative sensitivity   between   positive

diethylpropion

Penicillin G

samples was 100% . The relative specificity   between negative samples

The following compounds ilo not cross-react with (25 ng/ml cut-off)

Phencydidine at a 100 ng/ml concentration:

was 100%. The concordance of the combined data with respect to EMIT 11 was 100%.

acetaminophen

Ecgonine- HCL

phendimetrazine

Acetylsalicyclic Acid

Ecgonine Methyl Ester

Penicillin G

BIBLIOGRAPHY

amikacin

glucose

d- Propoxyphene

amitriptyline

histamine

l- Propanol

General

ampicillin

hydrocodone

phenobarbital

arterenol

hydromorhone

phentermine

1 ,     Baselt, R.C., Disposition of Toxic Drugs and Chemicals in Man, 2nd Ed., Biomedical   Publ.,   Davis, CA, p.488 (1982).

aspartame 

hydrochlorothiazide

phenylpropanolamine

Atropine Sulfate

indomethacin

l- Phenylephrine

2.     Cody, J.T., Schwarzhoff, R., J. Anal. Toxicol., 17: 2630 (1993).

Benzoic Acid

ketoprofen

quinine

3.     Urine Testing for Drugs of Abuse, NIDA Research Monograph 73, (1986).

Benzoylecgonine- HCL

levorphanol

ranitidine

caffeine

∆9- THC

Sodium salicylate

4.     Dasguspta, A,, Saldana, S., Kinnaman, G., Smith M., Johansen, K., Clin. Chem., 39(1):104-108  (1993).

chlorpheniramine

ll-Nor-∆9-THC-9-COOH

tryptophan

Chlorpromazine- HCL

meperidine

tetracycline

5.     Department of Health and Human Services, fed. Regist., 53(69): 11970-11989 (1988),    (1989).

cimetidine

methylphenidate

tetrahydrozoline

6.      FDA Guidance for Labeling Urine Drugs of Abuse Screening Testing, Kshitij Mohan,   7/21/1987.

deoxyephedrine

methadine

theophylline

7.     Blum, K., Handbook of Abusable Drugs, Gardner Press, Inc., New

dextromethorphan

methaqualaone

thioridazine

York, 1st Ed., (1984).

diazepam

Morphine-3-B-D-Glucuronide

trifluoperazine

8.     Tietz, N.W.: Clinical Guide to Laboratory Tests; W.B. Saunders Company, (1976). Cocaine  Test

diethylpropion

Morphine Sulfate

1.     D.W. Hoyt et al  J. Am. Med. Assoc., Vol. 258 (1987), pp. 504-509.

5,5-Diphenylhydantoin

oxazepam

2.     R.R. MacGregor, J.S. Fowler, and A.P. Wolf. J. of Chromatography, Vol. 590 (1992), pp. 354-58.

doxylamine

oxycodone

3,     E.J. Cone, D. Yousefnejad, and S.L. Dickerson    J. of Forensic Sciences, Vol. 35, No. 4 (1990), pp.   786-91.

4.     E.J, Cone et al. /. of Forensic Sciences, Vol. 34, No. 1 (1989), pp. 15-31

3.      Accuracy:   The accuracy of the QuikScreen*' 5   was first tested in urine samples in-house and subsequently in a clinical trial of   urine samples to a SAMHSA certified laboratory. In both cases the laboratories used Emit II as their screening procedure. All positive samples by either screening method were confirmed by GC/MS. The data was combined and  the results are summarized as follows:

5.     B. Holmstedt and A. Fredga. / of Ethnopharmacology, Vol. 3 (1981), pp.   113-47.

3. 1   amphetamine   (AMP)   l000 ng/ml Cut-Off Level

6.     T. Inaba and J, Can. Physiol. Pharmacoi, Vol. 67 (1989), pp.  1154-57

7.     A.R. Jeffcoat et al. Drug Metabolism and Disposition, Vol. 17, No. 2(1989), pp.  153-59.

                                         Syva EMIT II Positive      Svva EMIT II Negative

QuikScrecn   Positive                     205                                    0

QuikScreen    Negative                      0                                     237

When compared to EMIT II the relative sensitivity   between   positive

samples was 100% . The relative specificity  between negative samples was 100%. The concordance of the combined data with respect to EMIT 11 was 100%.